Abstract
To identify near complete sets of genes required for the cell cycle and cell shape, we have visually screened a genome-wide gene deletion library of 4843 fission yeast deletion mutants (95.7% of total protein encoding genes) for their effects on these processes. A total of 513 genes have been identified as being required for cell cycle progression, 276 of which have not been previously described as cell cycle genes. Deletions of a further 333 genes lead to specific alterations in cell shape and another 524 genes result in generally misshapen cells. Here, we provide the first eukaryotic resource of gene deletions, which describes a near genome-wide set of genes required for the cell cycle and cell shape.
Highlights
Understanding how cells reproduce and how they generate their shape are two major goals in eukaryotic cell biology
We have microscopically examined and described the deletion phenotypes of 4843 haploid gene deletion mutants of both essential and non-essential genes, after sporulating diploid heterozygous deletion mutants and germinating haploid spores on rich medium plates (see §5.1 for details of the screen and electronic supplementary material 1, tables S1– S3)
Mutants were classified to one of 11 cell shape phenotypes together with three additional categories, namely: WT; arrested as normal spores; or arrested as normal germinated spores, making 14 phenotype categories in total. These phenotypes were mapped to terms in the Fission Yeast Phenotype Ontology, and the gene list for each category can be found in the electronic supplementary material 1, table S5a –n
Summary
Understanding how cells reproduce and how they generate their shape are two major goals in eukaryotic cell biology. License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited. Given the importance of these two processes for cell biology, we have generated a genome-wide resource, cataloguing the genes that when deleted disrupt the cell cycle or cell shape in the fission yeast Schizosaccharomyces pombe. This is the first such resource that qualitatively describes a near complete set of genes required for these processes in a eukaryotic organism
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