Abstract
Modifications of the classical Edman cycle permitted the complete sequential degradation, without prior derivatization, of all small and medium-sized peptides with a free N-terminus. Sequences of up to 40 residues appeared possible with most long peptides. Quantities of 5–50 nmoles were optimal for the apparatus described. Cycling time was 50–90 min. Degradation was monitored and derivatives identified with a fast and sensitive thin-layer technique.
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