Abstract
SO2 derivatives and viscosity are important intracellular indicators, which are closely associated with various physiological metabolisms in organisms. The unregulated contents of SO2 derivatives and viscosity in vivo commonly related to some disorders. In this work, probe JFT was developed relying on FRET and TICT mechanisms for the simultaneous detection of SO2 derivatives and viscosity. JFT can rapidly detect viscosity levels with continuously enhanced fluorescence signals at 582 nm basing on the increasing of viscosity. Moreover, JFT was also sensitive to the changes of SO2 derivatives level with a low detection limit (61.5 nM), rapid responding time (with 16 min), excellent selectivity and anti-interference capacity. JFT could detect bisulfite in real water, wine and food samples with high accuracy and recovery rate. Cell imaging indicated that JFT could monitor the endogenous SO2 derivatives and viscosity in mitochondria. Importantly, JFT could recognize the cancer cells basing on the cell imaging difference of JFT in AGS and GES-1 cells.
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