Abstract
An apparatus for studying the mechanics of isolated frog heart myocytes is described. The cells are held horizontal in a through of Ringer solution by means of two suction micropipettes. Myocyte force is measured with an opto-electronic system recording the deflection of the tip of one micropipette, which acts as a cantilever force probe. The force probes are selected for compliance according to the force a myocyte is expected to develop in a given condition, so as to limit myocyte shortening during force development to no more than 1% of the slack cellular length (l0). The other micropipette, which is stiff relative to the forces measured, is mounted on an electromagnetic-loudspeaker motor by which controlled-velocity length changes, of preset size and in either direction, are imposed on myocytes. The force transducer has a sensitivity of 5-10 mV/nN, with a frequency response of 700-900 Hz in Ringer solution and a resolution of 0.5-1 nN. The motor with a suction micropipette can complete controlled-velocity length ramps within 1.5-2.0 ms, across a range of +/- 100 microns at a resolution of 8.0 nm. These values correspond, for frog-heart myocytes 200 microns and 400 microns long, to 25%-50% l0 and 0.002%-0.004% l0 respectively.
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