Abstract
A fluorometric technique for the determination of low concentrations of hydrogen sulphide in air streams is described and possible limitations examined. The method is particularly suitable where accurate measurements of these low hydrogen sulphide concentrations are required in small air sample volumes. The reagent is a simple solution of fluorescein mercuric acetate (FMA—biochemical laboratory reagent grade), in dilute aqueous sodium hydroxide. Hydrogen sulphide reacts rapidly with the alkaline FMA solution, causing a quenching of fluorescence which is related in a definite way to the amount of hydrogen sulphide contained in the air stream. Reagent stability, overall precision and reproducibility of the method were found to be very satisfactory.
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