Abstract
Hypochlorite anion is a ubiquitous reactive molecule in the terminal disinfection systems, inflammatory stress and immune systems. Thus, rapid and visual monitoring ClO− in water and biological samples is very meaningful for water quality safety and toxicity assessment of contaminants. Herein, a colorimetric and fluorometric probe (Rh-ClO) based on rhodamine B fluorophore and thiophene-2-carbohydrazide has been unveiled and successfully utilized for ClO− detection in water samples and HeLa cells. Upon addition of ClO−, color changes of solution from colorless to pink were immediately visible to the naked-eyes, meanwhile, brilliant red fluorescence was observed under excited at UV light (365nm). Rh-ClO displayed high selectivity and sensitivity for ClO−, and the detection limit was 7μmol/L calculated from the fluorescence titration. With the aid of its merits including rapid response to ClO− within 10s, Rh-ClO and its test paper could successfully detect ClO− in water. Additionally, HeLa cells image co-stained with Rh-ClO and Rh123 demonstrated that Rh-ClO possessed excellent and fast cell-membrane permeability and mitochondrion-targetability. It was clearly confirmed that Rh-ClO would be a promising probe for rapid tracking of ClO− in water samples and in mitochondria of living cells.
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