Synthesis and development of novel magnetic polymeric sorbent to simultaneous extraction of three anti-cancers using dispersive micro solid phase extraction procedure in biological and water samples

Abstract

A suitable sample preparation method based on dispersive micro solid-phase extraction was developed to extract and per-concentrate three anti-cancers, including Imatinib (IMT), methotrexate (MTX), and irinotecan (IRT), before their determination using HPLC-UV. A novel magnetic sorbent containing an aluminum-metal organic framework (CAU-1), magnetic Fe3O4 nanoparticles, and a polymeric component (Polyaniline) was prepared to extract anti-cancers. The sorbent composite was optimized using an optimal mixture design. The optimum value of CAU-1, magnetic Fe3O4 nanoparticles and polyaniline in the sorbent composite was 0.528, 0.338, and 1.13 g, respectively. Factors influencing the IMT, MTX and IRT determination were optimized using a two-stage experimental design strategy. The method has limit of detections in the concentration range of 0.06–0.33 ng mL−1 for the IMT, MTX and IRT determination in water and urine samples. The linear ranges for the IMT, MTX and IRT determination were 0.3–256, 0.2–297, and 0.4–289 ng mL−1 with R2 of 0.9923, 0.9931, and 0.9918 in water samples, respectively. The intra and inter-day RSDs for the IMT, MTX and IRT determination were between 3.9 and 5.2 and 4.6–6.2 % for analyzing water and urine samples, respectively. The sorbent provided good reusability of six cycles of extraction/desorption procedure without a significant decrease in the extraction recovery toward the anti-cancers. The method’s ability was investigated by analyzing urine and real water samples, indicating that the method exhibited a proper recovery and RSD % in the range of 88.0–95.8 % and 5.8–7.3 %, respectively.