Abstract
Biological species analyses on account of fluorescence detection technology are receiving increasing attention, because they combine the advantages both powerful detection capability and excellent imaging technology. By effectively integrating isophorone and phosphate group via p-hydroxybenzaldehyde, the alkaline phosphatase (ALP) detection probe was obtained. Based on the enzyme-catalyzed dephosphorization course, phosphate group was separated from the probe by ALP and released yellow fluorescence signal. Upon addition with ALP, the probe exhibited high selectivity, short response time (6 min) and longer emission peak shift (570 nm). Furthermore, bioimaging experiment results indicated that the probe could detect endogenous ALP effectively.
Published Version
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