Abstract
A novel fluorescence immunoassay based on MnO2 nanoflowers loading multicolor quantum dots and glutathione destroying MnO2 nanoflowers to release quantum dots combined with magnetic separation is developed for rapid, ultra-sensitive, and simultaneous quantitative detection of ochratoxin A, aflatoxin B1, fumonisin B1, and zearalenone in cereal samples. The test linear range of assay is from 0.001 to 200 μg L−1. The limit of detection for ochratoxin A, aflatoxin B1, fumonisin B1, and zearalenone is 0.0001 μg L−1, 0.0001 μg L−1, 0.0003 μg L−1, and 0.0001 μg L−1, respectively. The simultaneous detection of four mycotoxins can be achieve within 30 min. The test results of four mycotoxins in the incurred corn, rice, and oat samples have been confirmed by ultra-performance liquid chromatography tandem mass spectrometry, the differences between results are considered no significantly different (p > 0.05). This multiplexed test scheme has provided a potential analysis strategy for multiple food risk factors.
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