Abstract
The nitrogen-doped carbon dot (CDUN) was rapidly prepared by microwave method, using trisodium citrate as carbon source and urea as nitrogen source, and was characterized by fluorescence, absorption, resonance Rayleigh scattering (RRS), infrared and electron microscopy. The CDUN produced a fluorescence peak at 450 nm and a RRS peak at 375 nm, with an excited wavelength of 375 nm. When the aptamer (Apt) was added, the CDUN was encapsulated by Apt and reduced its fluorescence and RRS intensities. When K+ was added, it reacts with Apt to form a stable G-quadruplex and free CDUN. The fluorescence and RRS di-model intensity was enhanced linearly with the concentration of K+. Accordingly, a simple, rapid and sensitive di-model molecular spectral method for the determination of 0.008–0.27 μmoL/L K+ has been developed, with a detection limit of 0.3 nmoL/L.
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