Abstract

A field portable, multiwavelength, digital image processing system for the analysis of thin layer chromatography plates is described. The imaging module consists of a two-dimensional charge coupled device detector, a xenon strobe light source, a stepper driven filter wheel for wavelength selection, and hardware to digitize images. The instrument is controlled by a laptop computer resulting in a compact device the size of a 6-in. thick briefcase. As a representative application, software has been developed for the automated analysis of sulfa residues in pork products by thin layer chromatography (USDA SOS-test). Response to analyte concentration is linear over two and one half orders of magnitude with a correlation coefficient of 0.9998. Detection limit is 80 pg for sulfamethazine spotted on a plate.

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