A Fast Genetically Encoded Fluorescent Sensor for Faithful&amp;nbsp; &lt;i&gt;in vivo&lt;/i&gt;&amp;nbsp;Acetylcholine Detection in Mice, Fish, Worms and Flies

Philip Borden ,Abhi Aggarwal,Jonathan S Marvin,Dirk Dietrich,Yajun Zhang,Joseph Cichon,Jeremy S Dittman,Kaiming Guo,Joseph F Cheer,Baljit S Khakh,Masashi Tanimoto,Yuan Cai,Vivek Jayaraman,Chuntao Dan,Kaspar Podgorski,Xiaochu Lou,Li Lin,Mark A Lobas,Li Gan,Henry A Lester,Peng Zhang,Minoru Koyama,Guangfu Wang,Eiji Shigetomi,Huan Bao,Wen‐Biao Gan ,Amol V Shivange ,Edwin Chapman ,Jie Zhu ,Christopher P Ford ,Chengcheng Fan ,Andrew G Yee ,Douglas C Rees ,Hyun-Tae Kim ,Guang‐Xian Zhang ,Antonio Figueiredo ,Weitong Zheng ,Paula Zhu ,Ondřej Novák ,Benjie Xiang ,Loren L Looger

doi:10.2139/ssrn.3554080

A Fast Genetically Encoded Fluorescent Sensor for Faithful&nbsp; <i>in vivo</i>&nbsp;Acetylcholine Detection in Mice, Fish, Worms and Flies

Philip Borden , Abhi Aggarwal + Show 39 more

https://doi.org/10.2139/ssrn.3554080

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Journal: SSRN Electronic Journal	Publication Date: Jan 1, 2020
Citations: 6

Affiliation: Howard Hughes Medical Institute, Janelia Research Campus, University Hospital Bonn, University of Virginia, New York University, Cornell University, University of Mary, University of California, Los Angeles, University of Colorado Denver, University of Colorado Anschutz Medical Campus, University of Wisconsin–Madison, Wenzhou Medical University, California Institute of Technology, University of Yamanashi, Division of Chemistry, Thomas Jefferson University, Albemarle (United States)

#Bacterial Periplasmic Binding Protein #Decay Kinetics + Show 8 more

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Abstract

Here we design and optimize a genetically encoded fluorescent indicator, iAChSnFR, for the ubiquitous neurotransmitter acetylcholine, based on a bacterial periplasmic binding protein. iAChSnFR shows large fluorescence changes, rapid rise and decay kinetics, and insensitivity to most cholinergic drugs. iAChSnFR revealed large transients in a variety of slice and in vivo preparations in mouse, fish, fly and worm. iAChSnFR will be useful for the study of acetylcholine in all organisms.

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