Abstract
In this article, we demonstrated a facile method to prepare the porphyrin [5,10,15,20-Tetrakis (4-carboxyl phenyl)porphyrin] functionalized ZnS nanoparticles (H2TCPP-ZnS nanocomposites) under the mild conditions. Porphyrin molecules serves as both a functional molecule and stabilizer. TEM images showed that H2TCPP-ZnS nanocomposites are composed of nanoparticles with ca. 5–8nm in size. The nanocomposites were for the first time developed as a peroxidase mimetic with catalytic activities and could catalyze the colorimetric reaction of H2O2 and 3,3′,5,5′-tetramethylbenzidine (TMB), where the oxidized TMB showed a typical absorption peak at 652nm. Kinetic analysis indicated that the catalytic behavior was in accordance with typical Michaelis–Menten kinetics. Moreover, H2TCPP-ZnS nanocomposites could accelerate H2O2 to decompose into ·OH radicals. The intrinsic peroxidase-like activity of H2TCPP-ZnS nanocomposites is due to the synergistic effect of H2TCPP as well as ZnS in the nanocomposites. Based on these findings, a simple and sensitive colorimetric assay to detect H2O2 and glucose with a range of concentration from 0.01 to 0.06mM and from 0.005 to 0.5mM, respectively. In addition, this nanosensor can also be used for glucose detection in diluted serum.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call