Abstract
A colorimetric two-photon multianalyte sensor with spirobifluorene motif was developed. Along with the addition of hypochlorite/hydrazine to a solution of the probe, a colorimetric change from yellow to colorless and a fluorescence enhancement (Under 365 nm UV light) can be observed by ‘naked-eye’. The detection limits are 28 nM for hypochlorite and 12 nM for hydrazine, respectively. The molecule has large two-photon cross-section value of 220GM and has been successfully applied to two-photon imaging of hypochlorite / hydrazine in live cells. As far as we know, a two-photon fluorescent probe capable of detecting reactive oxygen species and reducing agent, and a colorimetric two-photon fluorescent probe for hypochlorite is reported for the first time. In addition, the detection limit for hydrazine is much lower than those of most hydrazine fluorescent probes reported in the literature.
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