Abstract
A dual-signal output plasmonic sensor based on glucose oxidase (GOx)-triggered etching of triangular silver nanoprisms (TAgNPRs) was developed for detecting fumonisins (FBs). In the sensor, glucose oxidase (GOx)-FB1 conjugation was used as competitive antigens that compete with FBs to bind with anti-FBs monoclonal antibody (McAb) coated in microplate well. GOx catalyzed glucose to produce hydrogen peroxide (H2O2) which acted as an oxidant to etch TAgNPRs. As a result, the wavelength of localized surface plasmon resonance (LSPR) peak blue shifted along with a color change of the solution. The amount of FBs was correlated to the wavelength shift of LSPR peak and the color change of the solution from light blue to violet in a linear range of 0.1–500 ng/mL. The colorimetric and LSPR detection limits were 0.1 ng/mL and 0.06 ng/mL respectively. The sensitivity of the dual-signal output sensor is better than those of aggregation plasmonic sensor and magnetoimmunosensor.
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