A dose-dependent effect of UV-328 on photosynthesis: Exploring light harvesting and UV-B sensing mechanisms

Abstract

Benzotriazole ultraviolet (UV) stabilizers (BUVs) have emerged as significant environmental contaminants, frequently detected in various ecosystems. While the toxicity of BUVs to aquatic organisms is well-documented, studies on their impact on plant life are scarce. Plants are crucial as they provide the primary source of energy and organic matter in ecosystems through photosynthesis. This study investigated the effects of UV-328 (2-(2-hydroxy-4′,6′-di-tert-amylphenyl) benzotriazole) on plant growth indices and photosynthesis processes, employing conventional physiological experiments, RNA sequencing (RNA-seq) analysis, and computational methods. Results demonstrated a biphasic response in plant biomass and the maximum quantum yield of PS II (Fv/Fm), showing improvement at a 50 μM UV-328 treatment but reduction under 150 μM UV-328 exposure. Additionally, disruption in thylakoid morphology was observed at the higher concentration. RNA-seq and qRT-PCR analysis identified key differentially expressed genes (light-harvesting chlorophyll-protein complex Ⅰ subunit A4, light-harvesting chlorophyll b-binding protein 3, UVR8, and curvature thylakoid 1 A) related to photosynthetic light harvesting, UV-B sensing, and chloroplast structure pathways, suggesting they may contribute to the observed alterations in photosynthesis activity induced by UV-328 exposure. Molecular docking analyses further supported the binding affinity between these proteins and UV-328. Overall, this study provided comprehensive physiological and molecular insights, contributing valuable information to the evaluation of the potential risks posed by UV-328 to critical plant physiological processes.