Abstract
This research was aimed to evaluate levels of genetic diversity and differences among populations of Malayan leaffish in the Mekong Delta. Fish samples were collected from three locations representing two types of habitats, including wetland conservation areas (Lang Sen-Long An and U Minh Ha-Ca Mau) and inland water bodies in Hậu Giang. Six inter-simple sequence repeats (ISSR) markers were used to amplify 95 samples. Results showed that 56 bands (5 to 12 bands per marker) were yielded with the polymorphic rate (P, %) of 86.9% and expected heterozygosity (He) of 0.250. Long An population (n=33) had the highest genetic diversity parameters (P = 98.2%; He=0.289), which were not significantly different (P>0.05) from those of the other two populations in Ca Mau (n=30; P=80.4%; He=0.239) and Hau Giang (n=32; P=80.4%; He=0.245). The three populations had high levels of genetic identity and a large number of migrant per generation (Nm=9.3). Analyses of Nei’s genetic distance and phylogenetic tree indicated Ca Mau and Hau Giang had a genetically closed relatonship, smaller than those between these two populations and Long An population.
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