A DNA biosensor strategy in monitoring of Vinorelbine breast cancer drug using catalytic effect of Pt–Pd–ZnO/SWCNTs

Abstract

The present research work introduced a new electrocatalyst (Pt–Pd–ZnO/SWCNTs in this case) to the fabrication of a powerful DNA biosensor in the monitoring of Vinorelbine anticancer drug. The characterization information confirms the high purity of Pt–Pd–ZnO/SWCNTs nanocomposite and an intercalation reaction between Vinorelbine anticancer drug and the guanine base of DNA in an aqueous solution. The reducing signal of DNA after interaction with Vinorelbine drug showed a linear analytical range of 0.1–120 μM with a detection limit of 0.05 μM. The biosensor was fabricated by layer-by-layer modification of glassy carbon electrode with ds-DNA and Pt–Pd–ZnO/SWCNTs nanocomposite and used as the working electrode to sensing of vinorelbine drug in pharmaceutical and other real samples with acceptable recovery data. The preferential intercalation mode for the binding of vinorelbine anticancer drug into the ds-DNA receptor is clarified using the molecular docking study.