A diterpenoid sugiol from &lt;i&gt;Metasequoia glyptostroboides&lt;/i&gt; with α-glucosidase and tyrosinase inhibitory potential

Sun Chul Kang,Vivek K Bajpai

doi:10.3329/bjp.v9i3.19026

Abstract

Nowadays use of plant derived natural compounds have become a topic of increasing interest in food and medicine industries due to their multitude of biological and therapeutic properties. In this study, a diterpenoid compound sugiol, isolated from Metasequoia glyptostroboideswas evaluated for α-glucosidase and tyrosinase inhibitory efficacy in terms of its potent anti-diabetic and anti-melanogenesis potential, respectively. As a result, sugiol at the concentration range of (100-10,000 µg/mL) and (20-500 µg/mL) showed potent efficacy on inhibiting α-glucosidase and tyrosinase enzymes in vitro ranging from 12.34-63.47% and 28.22-67.43%, respectively. These findings confirm the therapeutic potential of diterpenoid compound sugiol from M. glyptostroboides as a novel candidate for using in food and medicine industry which may have practical potential to cure skin and diabetes mellitus type-2 related disorders.

Highlights

The early stage of diabetes mellitus type 2 is associated with hyperglycemia due to impaired after-meal acute insulin secretion
Plant material The cones of M. glyptostroboides were collected from Pohang city, Korea, and identified by the morphological features and the database present in the library at the Department of Biotechnology, Daegu University, Korea
Fraction-14 was further purified by preparative thin layer chromatography (TLC) over silica gel GF254 using hexane-ethyl acetate (2:1) as a mobile phase to give one compound (122 mg) which on the basis of spectral data analysis was characterized as sugiol shown in Figure 1 (Bajpai et al, 2011)

Summary

Introduction

The early stage of diabetes mellitus type 2 is associated with hyperglycemia due to impaired after-meal acute insulin secretion. Hyperglycemia is believed to increase the production of free radicals and reactive oxygen species, leading to oxidative tissue damage and diabetic complications (Maritim et al, 2003). Glucosidase inhibitors reduce the rate of carbohydrate digestion and delay the carbohydrate absorption from the digestive tract. They have a potential to prevent the development of type 2 diabetes mellitus by lowering the after-meal glucose levels (Liu et al, 2011). Previous reports have shown that tyrosinase might be involved in neuromelanin production and be associated with Parkinson’s disease (Chen et al, 2013). Inhibiting tyrosinase activity is applicable to skin-lightening and in preventing neurodegeneration (Kwon et al, 2011)

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