Abstract
An alkali-soluble polysaccharide (Hunai polysaccharide, 1) from the fruit body of Pleurotus tuber-regium (Fr.) Sing was shown to be homogeneous by gel permeation chromatography and its molecular weight was ∼4.3×10 5. Complete acid hydrolysis, periodate oxidation, Smith degradation, methylation, FT-IR and 13C NMR analysis, complex formation with Congo Red, indicated that 1 has a β-(1→3)-linked d-glucopyranosyl backbone with a single β- d-glucopyranosyl group at O-6 of every third glucose residue.
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