Abstract
The applicability of a thermomorphic multiphasic system (TMS) composed of a hydrophobic deep eutectic solvent (DES) and an aqueous potassium phosphate buffer with a lower critical solution temperature (LCST) phase change for homogeneous biocatalysis was investigated. A lidocaine‐based DES with the fatty acid oleic acid as a hydrogen‐bond donor was studied. Phase diagrams were determined and presented within this study. We tested different additional components to the solvent system and observed a decrease in the cloud point of approximately 0.026 °C per concentration unit. Distribution studies revealed a clear distribution of the protein in the aqueous buffer phase (>95 %), whereas the hydrophobic substrate and educt accumulated (>95 %) in the DES‐enriched layer. Finally, a reduction catalyzed by horse liver alcohol dehydrogenase was performed in a larger‐scale experiment, and the biocatalyst could be recycled by simply removing the DES phase for three recycling runs.
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