Abstract
A potential fluorescent probe, N'-acetyl-2-((4-methyl-2-oxo-2H-chromen-7-yl)oxy)acetohydrazide (HMC1) was designed on the basis of photoinduced electron transfer (PET) and synthesized using cheap starting materials. HMC1 was found to be highly efficient as a Cu2+ ion quencher with a detection limit of 0.64 μM (~ 40 ppb). The binding mode of HMC1 towards Cu2+ was evaluated by 1H NMR, LC-MS and FT-IR techniques. In addition, cellular imaging studies further exhibited that HMC1 could be used an intracellular turn-off fluorescent chemosensor for Cu2+ in living cells.
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