Abstract
Quick and effective detection of biothiols in biological fluids has gained increasing attention due to its vital biological functions. In this paper, a novel reversible fluorescence chemosensor (L-Cu2+) based on a benzocoumarin-Cu2+ ensemble has been developed for the detection of biothiols (Cys, Hcy and GSH) in human urine. The chemosensing ensemble (L-Cu2+) contains a 2:1 stoichiometry structure between fluorescent ligand L and paramagnetic Cu2+. L was found to exclusively bond with Cu2+ ions accompanied with a dramatic fluorescence quenching maximum at 443 nm and an increase of an absorbance band centered at 378 nm. Then, the in situ generated fluorescence sluggish ensemble, L-Cu2+, was successfully used as a chemosensor for the detection of biothiols with a fluorescence “OFF-ON” response modality. Upon the addition of biothiols, the decomplexation of L-Cu2+ led to the liberation of the fluorescent ligand, L, resulting in the recovery of fluorescence and absorbance spectra. Studies revealed that L-Cu2+ possesses simple synthesis, excellent stability, high sensitivity, reliability at a broad pH range and desired renewability (at least 5 times). The practical application of L-Cu2+ was then demonstrated by the detection of biothiols in human urine sample.
Highlights
Biothiols, such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), play critical roles in cellular processes and are always involved in physiological and pathological actions [1,2,3]
2+ ensemble for based fluorescence chemosensor the selective detection of biothiolsfor under benzocoumarin-Cu based fluorescence chemosensor the simulated selective physiological detection of conditions and in human urine
Fluorescence chemosensor has been developed afornovel the detection of biothiols (Cys, Hcy(L-Cu and GSH)
Summary
Biothiols, such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), play critical roles in cellular processes and are always involved in physiological and pathological actions [1,2,3]. GSH, as the most abundant cellular biothiol, might cause oxidative stress and some other serious illness including cancers and AIDS [11,12,13] In view of their importance, developing safe, highly specific and sensitive detection methods for developing safe, highly specific and sensitive methods for biothiolsresearch in livingfields systems biothiols in living systems is strongly desired indetection biochemistry and biomedicine [14].is strongly desired in biochemistry and biomedicine research fields [14]. 2+ ensemble for based fluorescence chemosensor the selective detection of biothiolsfor under benzocoumarin-Cu based fluorescence chemosensor the simulated selective physiological detection of conditions and in human urine. As described in Scheme the fluorescent (L),in asScheme expected, biothiols under simulated physiological conditions and in1,human urine.
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