Abstract
The long-term maintenance of phytopathogenic viruses on potato and tobacco tissue culture plantlets was investigated. Several strains of PVYN, PVYO, PVX, PVA, PVM, PVS and PLRV have been kept in a tissue culture type collection of viruses at the Institut fur Kartoffelforschung Gross Lusewitz for 1–3 years. The serological and biological activity of cultures established in vitro of PVYN-D 884/Solanum tuberosum cv. Ackersegen, PVYO-1427/Nicotiana tabacum cv. Samsun, PVX-Erstling/Nicotiana tabacum cv. Samsun and PVX-Erstling/Solanum tuberosum cv. Arkula was proved.
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