Abstract
During heart development myofibrillogenesis and myocyte fiber pattern formation are critical steps in the genesis of a functional four chambered pump. The factors affecting these morphogenetic patterns are complex and include mechanical and chemical regulatory agents. The long range goal of this research is to model the effects of mechanical stimulation on heart development. In order to accomplish this goal, accurate images of the fiber patterns in the myocardium are necessary at various stages of development. In this study, we have used confocal scanning laser microscopy and immunofluorescence staining to visualize the fiber patterns and myofibrils in the developing rat heart. Sprague-Dawley rats at 10-15.5 days of gestation (Plug date was designated as day 0 with noon of plug date being designated as day 0.5) were sacrificed by lethal injection of anesthetic and their embryos were fixed in 2.0% paraformaldehyde in 0.1 M HEPES (PH 7.2) for 4-8 hours at 4°C.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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