Abstract
Myofibrillogenesis, myofiber pattern formation, and heart looping respond to physical, hormonal and teratogenic agents. In cell culture, myofibrillogenesis and myofiber pattern formation depend on coordinated expression of cytoskeletal and myofibrillar components and signals from extracellular matrix (ECM). However, these experiments have been conducted with isolated cardiac myocytes in which phenotype of myocytes is stellate rather than tubular as found in situ.Whole embryo culture (WEC) allows in situ interactions to occur during controlled application of perturbatory agents. Application of 10 μg/ml angiotensin to WEC results in 10% cardiac inversion (controls - 0% inversions). In this condition, hearts loop right, rather than left as in normal development. We are using confocal scanning laser microscopy (CSLM), transmission electron microscopy (TEM), and image reconstruction techniques to examine myofibrillogenesis and myofiber pattern formation in embryos allowed to develop in utero and WEC.Rat hearts 9.5 to 11.5 days in utero development, and hearts 9.5 days in utero and 48 H WEC were examined by CSLM and TEM.
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