A Comparitive Study of Detection of Serum Total and Direct Bilirubin by Jendrassik & Grof and Automated Method

Abstract

Background: Bilirubin is one of the major bile pigments, clinically and biologically important among the bile pigments present in the mammals. Material and Methods: The cross-sectional study was conducted in department of biochemistry in the collaboration with department medicine in Civil Hospital Tarn Taran. Out of 90 patients, 54 were males and 36 were females. Result: The comparison of Serum Total and Direct bilirubin by Jendrassik & Grof and Automated method was non-Significant with p value = 0.7788and 0.5394 respectively. The comparison of Serum Total and Direct bilirubin by Malloy Evelyn and Automated method is Highly Significant with p value =0.0002 and 0.0003 respectively. The mean difference of malloy with jendrassik & grof and automated was 2.394 and 2.162 respectively and the mean difference of jendrassik & grof with automated methodwas -0.231. Conclusion: It was concluded that a good correlation is found between bilirubin detection by manual and automated method. Non-significant mean difference between Jendrassik & Grof and Automated makesit better than Malloy Evelyn.