Abstract
Preservation of adipose tissue before the isolation of cells is one of the most important steps in maintaining the cell viability of adipose tissue-derived mesenchymal stem cells (ADSCs) for clinical use. Hank's balanced salt solution (HBSS) is one of the main ADSC preservation solutions used clinically. However, this step is known to lead to decreased cell viability. The University of Wisconsin (UW) solution is recognized by transplant physicians as an excellent organ preservation solution. We aimed to investigate the effectiveness of UW solution in preservation of the viability of ADSCs. We collected adipose tissue from the inguinal fat pad of mice and compared preservation in UW solution and HBSS overnight by measuring cell viability after isolation. We found that the number of viable cells harvested per gram of adipose tissue mass was higher in UW solution- than HBSS-preserved tissue.
Highlights
Mesenchymal stem cells (MSCs) [1] are a source of cell therapy in regenerative medicine
adipose tissue-derived mesenchymal stem cells (ADSCs) isolated without preservation served as the control group
Microscopy was performed to confirm the absence of abnormalities with regard to cell size and shape and the culture state in the Hank’s balanced salt solution (HBSS)- (Figure 4(a)) and University of Wisconsin (UW) solutionpreserved groups (Figure 4(c))
Summary
Mesenchymal stem cells (MSCs) [1] are a source of cell therapy in regenerative medicine. Among several sources of MSCs, adipose tissue-derived mesenchymal stem cells (ADSCs) [4, 5] have become a renowned therapeutic tool in clinical use [6,7,8]. Most clinical researchers using ADSCs follow their own particular protocol for isolating ADSCs, and there is no optimal storage medium for preserving adipose tissue after collection. There are several reasons why facilities that perform cell therapy with ADSCs use a preserving solution. It can take from several hours to as long as a day to transport the tissue from the operating room to the cell processing center. Some clinical researchers believe that overnight preservation using a mixture of storage medium and penicillin/streptomycin is necessary to prevent bacterial infection.
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