Abstract
To evaluate cytotoxicity of experimental conventional and resin modified glass-ionomer cements on UMR-106 osteoblast cell cultures and cell cultures of NIH(3)T(3) mouse fibroblasts specimens were prepared for every experimental material and divided into: group 1.Conventional glass-ionomer cements: GC Fuji IX GP Fast, GC Fuji Triage and Ketac Silver; group 2. Resin modified glass-ionomer cements: GC Fuji II LC, GC Fuji Plus and Vitrebond; group 3. Positive control was presented by specimens of composite Vit-l-ecence® and negative control-group 4. was presented by α-minimum essential medium for UMR-106 - osteoblast-like cells and Dulbecco's Modified Eagle's Medium for NIH(3)T(3) mouse fibroblast cells. Both cell cultures were exposed to 10% of eluate of each single specimen of each experimental material. Experimental dishes were incubated for 24 h. Cell metabolism was evaluated using methyltetrazolium assay. Kruskal-Wallis test and Tukey-Kramer post hoc test for the materials evaluated on NIH(3)T(3) mouse fibroblast cells, as well as UMR-106 osteoblast-like cells showed significantly more cytotoxicity of RMGICs, predominantly Vitrebond to both GICs and composite- Vit-l-ecence®.The lowest influence on cell's metabolism on UMR-106 osteoblas-like cells was shown by Ketac Silver and the lowest influence on cell's metabolism on NIH(3)T(3) mouse fibroblast cells was shown by Fuji IX GP Fast. Statistical evaluation of sensitivity of cell lines UMR-106 osteoblast-like cells and NIH(3)T(3) mouse fibroblast cells, using Mann-Whitney test, showed that NIH(3)T(3) mouse fibroblast cells were more sensitive for the evaluation of cytotoxicity of dental materials.
Highlights
Despite the fact that restorative dental materials and dental cements have improved their physico-chemical properties, level of intrinsic toxicity, or at least that part which appears in the evaluation in vitro remains rather high
Kruskal-Wallis test and Tukey-Kramer post hoc test for the materials evaluated on NIH T mouse fibroblast cells, as well as UMR- osteoblast-like cells showed significantly more cytotoxicity of resin modified glass-ionomer cements (RMGICs), predominantly Vitrebond to both glass-ionomer cements (GICs) and composite- Vit-l-ecence® .The lowest influence on cell's metabolism on UMR- osteoblas-like cells was shown by Ketac Silver and the lowest influence on cell's metabolism on NIH T mouse fibroblast cells was shown by Fuji IX GP Fast
Kruskal-Wallis test (Table .) and Tukey-Kramer post hoc test (Table .) for the materials evaluated on NIH T mouse fibroblast cells, shows significantly more cytotoxicity of RMGICs, predominantly Vitrebond (Z= - . , p< . )
Summary
Despite the fact that restorative dental materials and dental cements have improved their physico-chemical properties, level of intrinsic toxicity, or at least that part which appears in the evaluation in vitro remains rather high. It is necessary to reevaluate physical, chemical and biological properties of these dental materials, since it is obvious that they do not meet the requirements either from the standpoint of biosafety, or even from the standpoint of longevity of restorations When they emerged in the early seventies until today, glass-ionomer cements (GICs) are presented as biocompatible dental materials, with the possibility of chemical adhesion to tooth structure [ ], the ability to release fluoride [ ], ability to release-recharged-release fluoride [ ], Submitted 30 Jun 2012/ Accepted 29 September 2012 end enhance remineralization of caries lesion [ ]. It is believed that resin modified glass-ionomer cements combine the main advantages of glass-ionomer cements such as adhesion to tooth structure, fluoride release and biocompatibility, with easy handling of light polymerized composites [ ] They show some adverse properties when used as restorative materials, and the level of biocompatibility is not always satisfactory [ ]. Having in mind that both components of GICs, glass powder and polyacidic liquid (acrylic acid – itaconic acid or copolymer maleic and acrylic acid), were composed of spectrum of chemical formulas, risk that these materials
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