Abstract
Drug delivery via nanotechnology is one of the rapidly developing fields in cancer therapeutics. Targeted drug delivery has the advantage of having minimal interaction with healthy tissue, thereby reducing the toxicity of the drug to the rest of the body. rHDL nanoparticles are an efficient method of drug delivery for highly lipophilic anti-cancer drugs. Scavenger receptors class B type I (SR-BI), which are highly expressed on cancer cells interact with rHDL nanoparticles for effective drug delivery to the cancer cell and tumor. Valrubicin is an anti-cancer drug, with intrinsic fluorescence. In this experiment, we compared the photophysical properties of free valrubicin and rHDL valrubicin via steady state and time resolved fluorescence measurements. The steady-state anisotropy of rHDL valrubicin is higher as compared to free valrubicin, suggesting its encapsulation in rHDL nanoparticles. A longer rotational correlation time was observed for rHDL valrubicin in time resolved anisotropy measurements compared to free valrubicin, further supporting steady state anisotropy data.. We also studied the cellular internalization of free valrubicin and rHDL valrubicin using confocal microscopy. This could help track the movement of rHDL nanoparticles within the cancer cells.
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