Abstract
This study compared gradient gel electrophoresis (GGE) and light-scattering (LS) methods of determining low density lipoprotein (LDL) particle size. LDL was isolated from 27 fasting subjects. Peak particle size was determined by GGE on 3–13% gradient gels (Gradipore, Sydney, Australia) and by LS using a Zetasizer 3000 (Malvern Instruments, Malvern, UK). Repeated measurements on a single specimen indicated a coefficient of variation (CV) of 0.3%. A correlation was noted (P < 0.0001; r = 0.78) when comparing LDL particle size determined by LS methodology and GGE. Particle diameter results obtained by LS were smaller than those obtained by GGE (23.1 ± 0.1 vs. 26.1 ± 0.1 nm; P < 0.0001). LDL particle size determined by LS methodology correlated inversely with the log of triglyceride level (P < 0.0001; r = −0.77) and positively with high density lipoprotein (HDL) cholesterol level (P < 0.002; r = 0.57).—O'Neal, D., P. Harrip, G. Dragicevic, D. Rae, and J. D. Best. A comparison of LDL size determination using gradient gel electrophoresis and light-scattering methods. J. Lipid Res. 1998. 39: 2086–2090.
Highlights
This study compared gradient gel electrophoresis (GGE) and light-scattering (LS) methods of determining low density lipoprotein (LDL) particle size
LDL particle size measured by LS methodology on plasma samples that had been frozen was significantly larger compared to measurements performed on unfrozen samples (23.5 Ϯ 0.2 vs. 22.9 Ϯ 0.2 nm; P Ͻ 0.005)
While the difference in LDL particle size determined by LS methodology did not reach significance, a trend was observed towards larger diameters in the post-prandial state (23.9 Ϯ 0.2 vs. 23.5 Ϯ 0.2 nm; P ϭ 0.09)
Summary
This study compared gradient gel electrophoresis (GGE) and light-scattering (LS) methods of determining low density lipoprotein (LDL) particle size. Peak particle size was determined by GGE on 3–13% gradient gels (Gradipore, Sydney, Australia) and by LS using a Zetasizer 3000 (Malvern Instruments, Malvern, UK). A correlation was noted (P Ͻ 0.0001; r ؍0.78) when comparing LDL particle size determined by LS methodology and GGE. Particle diameter results obtained by LS were smaller than those obtained by GGE (23.1 ؎ 0.1 vs 26.1 ؎ 0.1 nm; P Ͻ 0.0001). LDL particle size determined by LS methodology correlated inversely with the log of triglyceride level (P Ͻ 0.0001; r ؍؊0.77) and positively with high density lipoprotein (HDL) cholesterol level (P Ͻ 0.002; r ؍ 0.57).—O’Neal, D., P. A comparison of LDL size determination using gradient gel electrophoresis and light-scattering methods.
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