Abstract
Cholesteryl ester transfer protein (CETP) plays a key role in reverse cholesterol transport and high density lipoprotein (HDL) metabolism. Predominance of small, dense LDL particles is associated with an increased risk of atherosclerosis and coronary heart disease (CHD).The aim of the study was to determine the potential relationship between the CETP concentration and low density lipoprotein (LDL) particle size and their association with intima media thickness (IMT) in patients with CHD. Lipid parameters, CETP concentration and LDL particle size were determined in 100 healthy subjects (control group) and in 100 patients with CHD, aged 43 to 77 years. Plasma CETP concentrations were measured by an enzyme-linked immuno-sorbent assay with two different monoclonal antibodies. LDL subclasses were separated by nondenaturing polyacrilamide 3-31% gradient gel electrophoresis. CETP concentration was higher in patients compared to controls (2.02 ± 0.75 mg/ml vs. 1.74 ± 0.63 mg/ml, p<0.01). Mean LDL particle size (nm) was significantly smaller in patients than in controls (24.5 ± 1.1 vs. 26.1 ± 0.9; p<0.001). There was no relation between LDL particle size and CETP concentration (r=-0.1807, p=0.072). Age, diastolic blood pressure, CETP concentration and LDL particle size were independent factors for determing IMT by multiple linear regression analysis. They accounted for 35.2 % of the observed variability in IMT. CETP is not an independent contributor of LDL particle size. CETP might play a role in determining lipoprotein distributions, but did not seem to be the sole factor in the formation of small LDL particles.
Highlights
Number of studies have demonstrated the association of decreased levels of high density lipoprotein (HDL) cholesterol and elevated levels of low density lipoproteins (LDL) with coronary heart disease (CHD) [ - ]
Since cholesterol ester transfer between lipoproteins plays a crucial role in intravascular LDL remodeling, we investigated the potential relationship between the Cholesteryl ester transfer protein (CETP) concentration and LDL particle size and association of plasma CETP concentration and LDL particle size with intima media thickness (IMT) in patients with CHD
systolic blood pressure (SBP) and diastolic blood pressure (DBP) were higher in CHD patients compared to control group
Summary
Number of studies have demonstrated the association of decreased levels of high density lipoprotein (HDL) cholesterol and elevated levels of low density lipoproteins (LDL) with coronary heart disease (CHD) [ - ]. LDL consists of a heterogeneous group of particles of varying size, density, electric charge, lipid and apolipoprotein composition [ , ]. Nondenaturing gradient gel electrophoresis is commonly used for separation of LDL particles by size. Griffin and colleagues showed that the predominance of small, dense LDL (sd LDL) particles is associated with an increased. Submitted: 26 February 2011 / Accepted: April 2011 risk of atherosclerosis and coronary artery disease (CAD) [ ]. The increased atherogenic potential of small LDL particles may result from their decreased binding to LDL receptors and increased binding to arterial wall proteoglycans [ ] or their increased susceptibility to oxidative modification [ , ]
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