Abstract
Six Manitoba soils of varying physical and chemical compositions were used to determine the efficiency of nitrate analysis by high-performance liquid chromatography (HPLC). The nitrate was extracted with distilled water and the extract was analyzed with a reverse phase column using a mobile phase of 1:1 methanol-water, pH 3.0. In five soils, the HPLC method of nitrate analysis resulted in near 100% recovery of added nitrate. An average 90.2% recovery was obtained with the hydrazine sulfate method using sodium bicarbonate, pH 8.5, as the soil extractant. Variable recoveries were obtained with the phenoldisulfonic acid method using a silver sulfate-copper sulfate extractant. Key words: HPLC, nitrate analysis, soil
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