A Comparison of Cytotoxic Effects of Mangifera Indica L. and Juglans RegiaAqueous Extract on Human Chronic Lymphocytic Leukemia.

Abstract

Natural products isolated from plant sources are well known for their pharmacological potential in diversity of disease treatments such as inflammatory or cancer conditions. Mango (Mangifera indicaL.) and Juglans regia are thought to be rich of functional phytochemicals. To clarify the anticancer activity, aqueous extracts of Juglans regia (JR) nut and Mangifera indicaL (MI) fruit were exanimated on chronic lymphocytic leukemia (CLL) B lymphocytes and their mitochondria and the results were compared with those of normal B lymphocytes. Cellular parameters such as viability and caspase 3 activity, and mitochondrial parameters such as reactive oxygen species (ROS), mitochondria membrane potential (MMP), mitochondrial swelling, and cytochrome c release were evaluated. Our results demonstrated that the extract of Mangifera indicaL increased cytotoxicity and caspase 3 activation through mitochondria pathway only in CLL B lymphocytes and also the extract of Juglans regia did not show cytotoxicity and caspase 3 activation on CLL and healthy B lymphocytes. Our in-vitro findings on isolated mitochondria indicated that mitochondrial ROS formation, MMP collapse, and mitochondrial swelling and cytochrome c release were significantly (p < 0.05) increased after addition of Mangifera indicaonly in cancerous mitochondria. These results demonstrated that Mangifera indicacan act as a promising source for anti-cancer drug candidates by directly and selectively targeting mitochondria and inducing selective mitochondria mediated apoptosis on CLL B lymphocytes.