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Abstract
Fusion of hepatocytes suspended in Krebs—Ringer medium without calcium ions induced by electric fields is compared here to fusion induced during centrifugation. The results obtained demonstrate clearly the possibility of fusing hepatocytes in a weak centrifugal field without making use of an external transient electric field or any fusogenic component. This procedure seems more efficient and versatile than electrofusion; indeed, similar fusion yields can be obtained with a better cell viability. A hypothesis is presented which might account for the fusion results in a centrifugal field.
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