A comparison between endogenous and exogenous lithium clearance in the anaesthetized rat

Abstract

The present study tests whether the endogenous renal lithium clearance (CLi) may be used as an alternative to the conventional method in order to avoid the adverse effects of exogenous lithium on renal function. Endogenous CLi) was measured by flameless atomic absorption spectrometry (AAS) in halothane anaesthetized, laparotomized rats and compared with values obtained after subsequent intravenous (i.v.) administration of LiCl. Endogenous plasma lithium concentration (PLi) was 0.30 +/- 0.06 mumol l-1. When measured by both conventional flame emission AAS and flameless AAS on the same samples, values for PLi did not differ significantly. Endogenous CL1/GFR was 0.195 +/- 0.01 (mean +/- SEM). Amiloride caused on significant change in either the endogenous CLi or 51Cr-EDTA clearance, a measure of glomerular filtration rate (GFR), but it caused a significant increase in CNa. Administration of exogenous Li+ had no significant effect on CLi (230 +/- 33 vs. 234 +/- 35 microliters min-1 gKW-1); both urine flow and CNa increased significantly (P < 0.01) (5.4 +/- 0.4 vs. 9.6 +/- 1.3, and 2.4 +/- 0.5 vs. 10.7 +/- 2.4 microliters min-1 gKW-1, respectively). GFR and calculated proximal tubular reabsorption rate (APR) both decreased by approximately 100 microliters min-1 gKW-1 (both P < or = 0.025), suggesting a moderate inhibition of APR after the acute elevation of plasma Li+ concentration. It is concluded that the endogenous CLi may be used as an alternative to the conventional method.