A comparative study on the biochemical effect of Chromolaena odorata and Calotropis gigantea on excised wound

Abstract

Wound healing is a dynamic, though intricate, process that involves the regeneration of tissue layers and any cells that have lost their vitality. Natural remedies derived from medicinal plants use multiple mechanisms to enhance healing and regeneration of the lost tissue. These remedies are considered to be an effective as well as safe alternative treatment for wounds. However, if these remedies are to be recommended for healing then the scientific validation, standardization and safety evaluation of plants becomes mandatory. In previous literature, it was found that parts of Chromolaena odorata and Calotropis gigantea was used asfolk medicinefor the various treatments including healing of wounds [Kavitha et al., 2017; [1,3,8]]. The wound healing potential of crude extracts of Chromolaena odorata and Calotropis gigantea leaves in albino rats was examined. On the back of each rat, full-thickness excision wounds were made, and latex was applied topically. Wounds in the experimental group were treated with C.odorata leaf juice and C.gigantea latex, while those in the control group were given the saline solution. The effectiveness of the treatment was examined based on significant increases in the amount of hydroxyproline content, hexosamine, total protein and uronic acid. The wound closure achieved at the end of 16 days was 99.36 % for the animal groups treated with leaf juice of C.odorata and 99.61 % for the animal groups treated with latex of C.gigantea. The effect of latex of Calotropis gigantea had increased levels of hydroxyproline content (1.052 mg), hexosamine (0.133 mg), total protein (139.25 mg) and uronic acid (7.685 mg) per 100 mg of the granulation tissue when compared with the levels of biochemical of the control treated wounds.