Abstract
Here, a novel biosensing platform for the detection of SARS‐CoV‐2 usable both at voltammetric and impedimetric mode is reported. The platform was constructed on a multi‐walled carbon nanotubes (MWCNTs) screen‐printed electrode (SPE) functionalized by methylene blue (MB), antibodies against SARS‐CoV‐2 spike protein (SP), a bioactive layer of chitosan (CS) and protein A (PrA). The voltammetric sensor showed superior performances both in phosphate buffer solution (PBS) and spiked‐saliva samples, with LOD values of 5.0±0.1 and 30±2.1 ng/mL, compared to 20±1.8 and 50±2.5 ng/mL for the impedimetric sensor. Moreover, the voltammetric immunosensor was tested in real saliva, showing promising results.
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