Abstract
A voltammetric immunosensor was developed to quantify a major peanut allergen, Ara h 1, using screen-printed carbon electrodes (SPCE) as transducers. A sandwich-type immunoassay was performed on nanodiamond-coated SPCEs using an alkaline phosphatase-labeled detection antibody and a mixture containing an enzymatic substrate (3-indoxyl phosphate) and silver nitrate. The immunological interaction was detected through the (linear sweep) voltammetric stripping of the enzymatically deposited silver. The immunosensor’s applicability was evaluated by analyzing breakfast cereals, cookies, and energy and cereal bars. Ara h 1 was successfully tracked in these commercial food products.
Highlights
Peanuts are integrated into the Mediterranean dietary pattern, and their consumption has been recommended worldwide
Few electrochemical immunosensors were reported for the determination of Ara h 1: a sandwich-type gold nanoparticle-coated screen-printed carbon electrodes (SPCE) [3], a reagentless label-free single-walled carbon nanotube-based biosensor [4] and an impedimetric immunosensor using a gold electrode functionalized with 11-mercaptoundecanoic acid self-assembled monolayer [5]
The present work reports the development of an electrochemical immunosensor for the analysis of the peanut allergen Ara h 1 using SPCEs/NDs
Summary
Peanuts are integrated into the Mediterranean dietary pattern, and their consumption has been recommended worldwide. Few electrochemical immunosensors were reported for the determination of Ara h 1: a sandwich-type gold nanoparticle-coated SPCE [3], a reagentless label-free single-walled carbon nanotube-based biosensor [4] and an impedimetric immunosensor using a gold electrode functionalized with 11-mercaptoundecanoic acid self-assembled monolayer [5]. Among the distinct carbon-based nanomaterials, nanodiamonds (NDs) have not yet been used in the analysis of allergens. Due to their 3D configuration efficient electrode nanostructuration can improve the analytical signal [6]. The present work reports the development of an electrochemical immunosensor for the analysis of the peanut allergen Ara h 1 using SPCEs/NDs. In a sandwich-type assay, the antibody–antigen interaction was detected through Linear Sweep Voltammetry (LSV)
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