Abstract
Thromboxane B 2, 6-keto-Prostaglandin F 1α, and Prostaglandin E 2 release have been quantitated from cultured adult by bovine endothelial cell monolayers and from ex Vivo vascular segments employing specific radioimmunoassay and thin layer chromatography. Release of all three prostaglandins was demonstrable from both endothelial cell systems under basal conditions and following exposure to the ionophore A23187 and arachidonic acid. In culture, the quantity of 6-keto-PGF 1α released was diminished compared to amounts released from the vessel segments while thromboxane B 2 and prostaglandin E 2 release were similar in the two endothelial model systems. However, the amount of thromboxane B 2 assayed was small and the quantity of thromboxane A 2 it represents is probably of little in Vivo significance to prostacyclin.
Published Version
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