Abstract
Dark fixation of 14CO2 was followed in potato disks under varying salt treatments at 0° C and 25° C. It is shown that the specific activity of the 14CO2 supplied is heavily diluted by endogenously produced CO2 and that the apparently greater fixation of 14CO2, at 0° C as compared with that at 25 ° C is due to the lower respiration rate at 0° C, with consequently less dilution of the 14CO2. supplied. At 25° C organic acid formation in response to different salt treatments fulfils the common expectation, 14CO2 fixation increasing in the presence of K2SO4 and decreasing in CaCl2 relative to that in KCl. The role of organic acids in maintaining ionic balance within the cell at 25° C is thereby indicated but at 0° C organic acid adjustments did not follow the normal pattern. At 25° C but not at o° C increasing external concentration of KCI resulted in an increased level of 14CO2 fixation.
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