Abstract
Photobiomodulation (PBM) is a clinically accepted tool in regenerative medicine and dentistry to improve tissue healing and repair and to restore the functional disability. The current in vitro study aimed to investigate the photobiomodulatory effects of 980 nm wavelength (the real energy at the target: ~0.9 W, ~0.9 W/cm2, 60 s, ~55 J/cm2 and a single energy ~55 J in CW) on MC3T3-E1 pre-osteoblast, delivered with flattop profile in comparison to the standard profile. The laser groupings and their associated energies were: Group 1 - once per week (total energy 110 J); Group 2 - three times per week (alternate day) (total energy 330 J); Group 3 - five times per week (total energy 550 J). The metabolic activity and the osteoblasts maturation were analyzed by alkaline phosphatase assay, alizarin red S histological staining, immunoblot and/or double immunolabeling analysis for Bcl2, Bax, Runx-2, Osx, Dlx5, osteocalcin, and collagen Type 1. Our data, for the first time, prove that laser irradiation of 980 nm wavelength with flat-top beam profile delivery system, compared to standard-Gaussian profile, has improved photobiomodulatory efficacy on pre-osteoblastic cells differentiation. Mechanistically, the irradiation enhances the pre-osteoblast differentiation through activation of Wnt signaling and activation of Smads 2/3-βcatenin pathway.
Highlights
Bone tissue regeneration remains a challenge in the field of oral and maxillofacial surgery reconstruction
Consistent with the standard error of the instrument, the powermeter measurements in contact mode showed that the power output provided by the hand-pieces (ST vs. FT) were similar (P < 0.05) and in accord with the 980 nm laser set-up (∼1 W) (Table 1B, output energy, contact)
The effective power output reached the MC3T3-E1 pre-osteoblast cells was 0.80 ± 0.02 W and 0.93 ± 0.02 W (ST vs. FT) (Table 1B, coming down energy– air, αMEM, 3 mm), which showed a decrease by −20 and −7%, respectively in comparison to the 1 W power output set up on the laser device
Summary
Bone tissue regeneration remains a challenge in the field of oral and maxillofacial surgery reconstruction. Effectiveness of 980 nm Photobiomodulation on Osteoblasts Maturation in containing osteogenic cells and bone matrix proteins, which support the bone growth [2, 3]. They are associated with post-operative complications in terms of donor morbidity, which has been estimated approximately 30,000 patients per year worldwide [1]. PBM is a clinically accepted tool in regenerative medicine and dentistry to improve tissue healing and repair and restore the functional disability [3,4,5]. Evidence based-medicine has shown that PBM carries a minimal to none adverse effects on patients’ health status in comparison to other tissue regenerative modalities [3, 5, 6]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
