Abstract
A Comparative Study between Hydrogen Peroxide Amperometric Biosensors Based on Different Peroxidases Wired by Os-Polymer: Applications in Water, Milk and Human Urine
Highlights
In the last few years, hydrogen peroxide (H2O2) real-time monitoring has attracted great interest due to its large employment in industrial processes: in particular, H2O2 is released into the environment in small or large amounts, since it is used as an oxidant, bleaching and sterilizing agent [1]
A comparison has been made between two plant peroxidases, cationic horseradish peroxidase (HRP) and anionic tobacco peroxidase (TOP) [3], wired by a cationic osmium polymer ([Os(dmp)PVI]+/2+) [4,5] to prepare highly sensitive, stable and selective hydrogen peroxide biosensors
HRP and TOP peroxidases were immobilized onto graphite rod (G) electrodes by a simple drop-casting procedure using a solution of poly(ethyleneglycol) diglycidyl ether (PEGDGE), as cross linking agent
Summary
In the last few years, hydrogen peroxide (H2O2) real-time monitoring has attracted great interest due to its large employment in industrial processes: in particular, H2O2 is released into the environment in small or large amounts, since it is used as an oxidant, bleaching and sterilizing agent [1]. H2O2 has been found as a valuable biomarker present in human urine which may elucidate specific levels of oxidative stress in vivo [2].
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