Abstract
In numerous biological activities, 3-(1-Naphthyl)-D-alanine Hydrochloride (D-1NA) serves as an essential metabolic precursor and amino acid receptor. Therefore, a sufficient supply of D-1NA is very much important. Here, we look into how D-1NA interacts with the hydrophobic cavity of β-cyclodextrin (BCD) to form the host–guest assembly. Analysis of experimental and theoretical findings demonstrates the creation of a stable IC, which is suggestive of the usefulness of BCD as a delivery method for D-1NA. The binding behaviour of this newly created 1:1 inclusion system of D-1NA with BCD has been examined by UV–visible spectroscopy and discovered that BCD showed a considerable affinity for D-1NA and the binding process was thermodynamically possible. Molecular docking analysis demonstrated the most reliable D-1NA binding orientation inside the BCD, as shown by Fourier transforms infrared and 1H-Nuclear magnetic resonance spectroscopic analyses. Studies using PXRD and SEM images also showed that inclusion complexes had formed. After complexing with BCD, D-1NA's photostability is increased. Additionally, it was described how the produced IC is bound to the Deoxyribonucleic acid (DNA) of the calf thymus (CT).
Published Version
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