Abstract
The mixed-bed resin column charged with dithionite is shown to be an efficient deoxygenator. Deoxyhemoglobin prepared on this column from stock oxyhemoglobin in solution has normal spectral characteristics, and upon reoxygenation the oxyhemoglobin spectrum is identical with that of the original stock oxyhemoglobin solution. The deoxyhemoglobin solution has a pO 2 of less than 1 mm Hg and an ionic strength of less than 5 × 10 −5, and exhibits a Bohr effect and deoxygenation kinetics consistent with literature values. Deoxygenated water is also prepared by this method. Dithionite, oxidation products of dithionite, and ionic contaminants in the elluent remain bound to the resin.
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