A Clinical, Immunohistochemical, Doble Fluoresce Immunostaining and Ultrastructure Analysis of Papillary Craniopharyngioma

Abstract

Background: Craniopharyngioma is epithelial tumor located in the parasellar region, classified into histopathologically, genetically, clinically and prognostically as adamantinomatous and papillary subtypes Aim: A descriptive design, to determine the immunohistochemical expression of BRAFV600E, β-catenin, E-cadherin, clau1- 5, occludin, TNFα, IL6,10,20, gal-1,3,9 and Glut-3, 4, βFGF, IGF, EGFR, DPGF, Ki-67li and MVD in PaCPs. Materials and Methods: clinic, pathological immunostaining and ultrastructure correlation on paraffin embedded tissue sections of 13 cases of papillary craniopharyngioma. Results: Results. 10 (77%) male and 3 (23%) females, 8 (62%) recurrence and 5(39%) did not. 6 had a cystic appearance, 6 with atypia, 7 with squamous metaplastic changes with foamy macrophages and inflammation. Those who recurred presented higher indices of Ki67 and MVD, CK7, glut3, Gal3, Il6, Il20, and CD68 overexpression. BRAFv600E was doble immunofluorescence coexpresed with Il6, β-catenin, and TNFα as well as lower expression of E-cadherin, β-catenin, occludin, than those who did not recur. Likewise, by EM vacuole binding proteins, glycogen deposits and an increase in microgranules and loss of desmosomes observed. Opening or loss of intercellular adhesions. Pa CPs also presents paracrine secretion and probably lead to OMF production. Discussion: We observed a correlation with the expression of BRAFV600E with higher Ki-67Li and MVD, strong expression of growth factors, glucose transport galectins, inflammatory factor and loss of membranal TJ proteins expressed in epithelial cells. With they play an important role in cell polarity and cell adhesion, brain invasion as well as, in maintaining paracellular barrier functions, paracrine secretion and senesce.