A chromosome-scale draft genome sequence of horsegram (Macrotyloma uniflorum).

Kenta Shirasawa,Hideki Nagasaki,Soichiro Nagano,Sachiko Isobe,Rakesh Chahota,Tilak Sharma,Hideki Hirakawa

doi:10.46471/gigabyte.30

Abstract

Horsegram (Macrotyloma uniflorum [Lam.] Verdc.) is an underutilized warm-season diploid legume (2n =20, 22). Because of its ability to grow under water-deficient and marginal soil conditions, horsegram is a preferred choice in the era of global climate change. In recognition of its potential as a crop species, we generated and analyzed a draft genome sequence for a horsegram variety, HPK-4. Ten chromosome-scale pseudomolecules were created by aligning Illumina scaffold sequences onto a linkage map. The total length of the ten pseudomolecules was 259.2Mbp, covering 89% of the total length of the assembled sequences. A total of 36,105 genes were predicted on the assembled sequences. Diversity analysis of 89 horsegram accessions by dd-RAD-Seq identified 277 single nucleotide polymorphisms (SNPs), suggesting narrow genetic diversity among the horsegram accessions. This is the first attempt to generate a draft genome sequence of horsegram and will provide a reference for sequence-based analysis of horsegram germplasm.

Highlights

Horsegram (Macrotyloma uniflorum [Lam.] Verdc.) (NCBI:txid271171), is an underutilized warm-season diploid legume (2n = 20, 22)
Because of its ability to grow under water-deficient and marginal soil conditions, horsegram is a preferred choice in the era of global climate change
To elucidate the potential of this food legume species, we generated and analyzed a draft genome sequence for HPK-4, a horsegram cultivar commercially released by CSK Himachal Pradesh Agricultural University (HPAU), Palampur, India

Summary

Background

To elucidate the potential of this food legume species, we generated and analyzed a draft genome sequence for HPK-4, a horsegram cultivar commercially released by CSK Himachal Pradesh Agricultural University (HPAU), Palampur, India. This variety, which has dark-brown seeds, is under cultivation in many parts of the Northwestern Indian Himalayan region. It is resistant to anthracnose (Colletotrichum truncatum) and tolerant to abiotic stresses such as drought, salinity, and heavy metals This is the first attempt to generate a draft genome sequence of this ‘orphan’, but it is an important food legume species and will provide a reference for sequence-based analysis of horsegram germplasm to elucidate the genetic bases of important traits

METHODS

Findings

Platanus