Abstract
The association between intestinal flora and ulcerative colitis (UC) was studied in order to provide a basis and method for clinical treatment. Fresh fecal samples were collected from 30 active UC patients and 10 healthy controls. The intestinal flora DNA from each sample was extracted and 16S rRNA gene sequencing was carried out using HiSeq platform to identify the intestinal flora in fecal samples. The richness and diversity of intestinal flora in UC patients were significantly lower than those in healthy control group (P < 0.05). Significant differences were observed between the intestinal flora-species of UC patients and healthy controls. Synergistetes (P < 0.01) and Firmicutes (P < 0.05), along with probiotics Veillonella (P < 0.01), Ruminococcus and Coprococcus (P < 0.05) in the UC patients were lower than that in the healthy controls significantly. Furthermore, compared with the control group, Tenericutes (P < 0.01) and intestinal pathogenic bacteria, including Bacteroides (P < 0.01), Escherichia and Sutterella (P < 0.05) were significantly increased. The incidence of UC is significantly associated with the changes in intestinal flora. Changes in intestinal flora may lead to a decrease in the diversity of intestinal flora or to the enrichment of a particular intestinal flora.
Highlights
Ulcerative colitis (UC) is a chronic non-specific intestinal inflammatory disease of unknown etiology
In this study, a case–control experimental method was adopted and 16S rRNA gene sequencing technology using Illumina HiSeq 2500 sequencing platform was applied to explore the relationship between changes in intestinal flora of UC patients and healthy controls
The results showed that the species richness and diversity of the intestinal flora in the UC patients were significantly lower than those of healthy controls
Summary
Ulcerative colitis (UC) is a chronic non-specific intestinal inflammatory disease of unknown etiology. Some UC patients have extra-intestinal manifestations and complications, which affect their daily lives, and even their health (Whitehead 2016; Sykora et al 2018). An epidemiological survey reported higher prevalence of UC among the people of 20–40 years, and higher prevalence in males than females (Lewin et al 2019). The incidence rate of UC fluctuates from 5.5 to 24.3 per million people worldwide with significant regional and ethnic specificity. The prevalence of UC in China is about 11.6 per million (Whitesides 1985; Molodecky et al 2012; Ng et al 2013; Cui et al 2018). The pathogenesis of UC has been reported to be associated with
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