Abstract
Capillary zone electrophoresis (CZE) of serum proteins is a well-accepted method used in clinical chemistry laboratories to separate serum proteins and detect monoclonal (M) proteins. The detection is based on ultraviolet absorbance measurements at 200 nm (Capillarys, Sebia) or at 214 nm (Paragon 2000, Beckman Coulter), which correspond to the absorption of peptide bonds in proteins. Compared with gel electrophoresis, CZE offers the advantage that all proteins are quantified. Most interferences on CZE are caused by exogenous nonprotein substances that also absorb at 200/214 nm, such as radioopaque agents and antibiotics (1). Gelatin-based plasma expanders have also been reported to cause interference. Gay-Bellile and Gijbels reported an increase in the β/γ-region on CZE in samples from patients who had received infusions with …
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