Abstract
In this work, an electrochemical assay for 3-chloro-1,2-propandiol (3-MCPD) in soy sauce was successfully developed that made use of cytochrome-c (Cyt-c). The synthesized gold–silver nanostars (Au@AgNSs) were deposited on a Fe-based metal–organic frameworks (FeMOF)-coated gold electrode (AuE). The obtained Au@AgNSs/FeMOF/AuE was used as a host matrix for the covalent immobilization of Cyt-c. The direct electrochemical behavior of Cyt-c on the Au@AgNSs/FeMOF-modified electrode and its application as 3-MCPD biosensor was investigated. The results indicated that Cyt-c was successfully adsorbed on the electrode surface and exhibited enzymatic catalytic activity for the oxidation of 3-MCPD. Cyclic voltammetry (CV) measurement showed that the current response of Cyt-c/Au@AgNSs/FeMOF/AuE was 5.7 times higher than that of Au@AgNSs/FeMOF/AuE to the equivalent concentration of 3-MCPD. Under optimal conditions, the CV signal from the biosensor was linearly related to 3-MCPD concentrations from 2 to 100 mg L−1, and the limit of detection was 0.41 mg L−1. The utility of the sensor was verified in actual samples, and an acceptable recovery was obtained.
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